Chelsea take on Spurs this afternoon, with the two sides preparing to play their first ever Premier League game at the national stadium.
And Conte, speaking to the Daily Mail, believes the loss of home comforts will take its toll on Spurs with the club forced to play all their matches this season at Wembley.
The Chelsea boss experienced something similar at former side Juve, as the Italian giants moved from its former ground, Stadio delle Alpi, to the Juventus stadium.
“For sure it’s not the same when you don’t play in your stadium. It’s different. But if you want to have another good step to improve the club, you must have this type of situation,” he said.
“Moving to a new stadium was a key moment for Juventus. I think there is a big difference in the new stadium because the delle Alpi in which I played a lot, wasn’t a real stadium.
“It was very cold and you don’t feel the passion of the fans because the stand was very far from the pitch.
“When we started to play in Juventus Stadium, the situation changed totally, above all for the players. The players felt the fans behind them. It’s great.”
Chelsea are set to follow in their London rivals’ footsteps, with Stamford Bridge due for redevelopment in the next few years and Conte believes it is a positive move for the club.
“Stamford Bridge is similar to Juventus Stadium and is a really good stadium, but for sure the new stadium will be fantastic for the club, for the coach, for the players.”
Ahead of the clash at Wembley, Conte has several team selection headaches with Cesc Fabregas and captain Gary Cahill suspended.
Victor Moses is back following a ban, but Chelsea will be hoping Pedro and summer signing Tiémoué Bakayoko come through fitness tests. The game is likely to come too soon for Eden Hazard.
Spurs come into the game with no new injury worries from last weekend’s win at Newcastle.
Erik Lamela and Danny Rose remain on the sidelines, but Kieran Trippier could play a part after recovering from an ankle problem quicker than expected.